C2c12 myotube cells
WebJun 10, 2024 · Myotube Analyzer functions. Users perform the analysis using the app step-by-step. An instruction manual, a detailed definition of all outcomes and an example analysis can be found in the GitHub repository [].The output of the app is saved in the same folder as the input images and consists of several images saved as PNGs in different steps of the … WebSep 1, 2010 · C2C12 myotube. Contractility. Electrical pulse stimulation. ... Murine C2C12 cells (less than six passages; American Type Culture Collection, U.S.) were maintained in a 37 °C, 5% CO 2 incubator and cultured in a growth medium: DMEM containing 10% FBS, 100 U mL −1 penicillin and 100 μg mL −1 streptomycin [10], [11]. When cell densities ...
C2c12 myotube cells
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WebApr 11, 2024 · The C2C12 cells were cultured in DM for 5 days, ... The cell nuclei were stained blue by the DAPI, and the myotube cells were stained red due to the presence of MyHC and Alexa Flour 594. WebSpontaneous and repetitive calcium transients in C2C12 mouse myotubes during in vitro myogenesis ... i spikes) and (iii) [Ca2+]i spikes detectable in the entire myotube (global [Ca2+]i spikes). [Ca2+]i oscillations and localized [Ca2+]i spikes were detectable following the appearance of caffeine-sensitivity in differentiating C2Cl2 cells ...
WebMar 11, 2024 · Myotubes derived from C2C12 cells or primary myoblasts that were cultured on murine laminin formed numerous large AChR clusters at ... Myotube differentiation was induced by gentle replacement of ... WebI've been working on c2c12 differentiation using this protocol. 1. Switch to diff. medium (DMEM, 2% horse serum, 1% penicillin/streptomycin) after reaching full confluence …
WebMar 16, 2024 · Consistent with the results of the 2D cell culture , myotube width of C2C12 cells in 3D tissue constructs was significantly increased by TSA (0.01 and 0.1 μM), VPA (0.1 and 1 mM), SB (0.5 and 5 ... WebAug 15, 2011 · Mammalian cells are sensitive to the physical properties of their micro-environment such as the stiffness and geometry of the substrate. It is known that the stiffness of the substrate plays a key role in the process of mammalian myogenesis. ... C2C12 myotube alignment on the micropatterned islands. Controlling the alignment of …
WebFeb 5, 2024 · C2C12 cells were washed with phosphate buffered saline (PBS) (pH7.4) and fixed with 90% methanol at −20 °C for 10 min. After fixation, the cells were incubated in 5% milk in PBS for 30 min at 37 °C for blocking. Cells were incubated at room temperature with MF-20 (primary antibody) diluted in blocking buffer (5% milk PBS) for 1 h.
WebMethods: Mouse myoblast C2C12 cells were differentiated for 5 days to form myotubes. Sodium lactate (lactate) or GPR81 agonist, 3,5-dihydroxybenzoic acid (3,5-DHBA), was administered to the differentiation medium. ... (p90RSK). MEK inhibitor U0126 depressed the phosphorylation of ERK-p90RSK and increase in myotube diameter induced by … how to embed code into powerpointWebApr 30, 2024 · To exclude the effect of the C2C12 myotube differentiation protocol, we repeated the experiment by using another differentiation method (2% HS), demonstrating that the decreased responsiveness of the C2C12 cells to myostatin after differentiation is independent of the differentiation protocol (Supplementary Material Figure S4). how to embed data in excelWebC2C12 Cell Differentiation is Ongoing at 7 days. C2C12 cells were differentiated as previously described. The mean ± SEM of (A) myotube number, (B) myotube nuclei, (C) myotube length, (D) myotube ... how to embedded video in powerpointWebMar 16, 2024 · However, following differentiation, myotube cytoplasm appears dark purple (Figure 1F; arrowheads), while lighter nuclei are clearly visible within the multinucleated cell ... C2C12 cells were differentiated for 5 days in the presence or absence of 10 µM BB94 (Cat. #ab142087; Abcam, Cambridge, UK) and subsequently fixed and stained with … how to embedded documents in wordWebWe examined the myofibril biochemical, structural, and biophysical properties of C2C12, a mouse skeletal muscle cell line (American Type Culture Collection), to assess whether … how to embedded excel in powerpointWebMar 10, 2024 · C2C12 cells were cultured in 96-well plates for 24 h, then treated with 100 μM PA or vehicle control in triplicate as described in Materials and Methods.The effect of … led ice shanty lightsWebJan 5, 2024 · Under high serum conditions, teaghrelin inhibited C2C12 cell proliferation, indicating a cell cycle arrest and cessation of proliferative progression. Teaghrelin promoted pro-differentiation of C2C12 cells as evidenced by a progressively elongated morphology, as well as the induction of muscle specific myogenin, myosin heavy chain (MHC), and … ledicias wedding planner