Jc 1 staining principle
WebJC-1) to green fluorescence (JC-1 monomers). This kit enables a fast and simple staining of isolated mitochondria from animal tissues and cell lines. It includes valinomycin, a mitochondrial membrane-dissipating agent, for control experiments. Components The kit is sufficient for 50 staining assays of 2 ml using JC-1 Stain 25 g WebPrepare Staining Solution. Mix 25 µl of the 200× JC-1 Stock Solution in 4 ml of ultrapure water in an appropriate tube. Close the tube and mix the solution by inversion or vortex …
Jc 1 staining principle
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WebOn the basis of this phenomenon, several fluorescent probes, including rhodamine 123 and JC-1, have been developed to monitor the function of mitochondria in living cells (Smiley et al., 1991; Johnson et al., 1980, 1981). WebThe cyanine dye [ 1] JC-1 has been used to determine mitochondrial transmembrane potential. [ 2] [ 3] [ 4] A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λ ex 520 nm) monomer at low membrane potential.
WebJan 1, 2024 · JC-1 can be used to assess the ΔΨ M both in intact isolated mitochondria and tissues. In fact, the JC-1 dye is a lipophilic, cationic dye (naturally exhibiting green … WebFurthermore, another kind of dye, JC-1, yields green or red fluorescence depending on MMP and allows to distinguish cells with high or low mitochondria potential. Both measuring …
Web(B) MitoProbe JC-1 Assay Kit was used to stain Jurkat cells (T-cell leukemia, human) which were then analyzed on a flow cytometer using 488 nm excitation with 530 nm and 585 nm bandpass emission filters. Green = apoptotic cells (reduced mitochondria membrane potential), red = normal cells. TOP MitoProbe DiOC 2 (3) Assay Kit WebJC-1 (CBIC2) is an ideal fluorescent probe widely used to detect mitochondrial membrane potential. JC-1 accumulates in mitochondria in a potential dependent manner and can be used to detect the membrane potential of cells, tissues or purified mitochondria.
WebIt changes to monomeric form and stains cells in green fluorescence. Although JC-1 is widely used in many labs, its poor water solubility causes great inconvenience. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 is developed to be a superior alternative to JC-1 when high dye concentration is desired.
WebRemove and incubate for 5 min at 20–22°C. The reaction is initiated by the addition of 50 μL of the dye reagent and allowed to proceed for 30 min. The incubation mixture is then filtered on a 0.2 µm pore filter either through a syringe filter unit or an inserted filter for microcentrifuge tube as shown in Figure 7.1. free sign language sheets to printWeb1.3 1 mM JC-1 Stock Solution: Allow the lyophilized vial JC-1 and the DMSO to warm to room temperature. Reconstitute the JC-1 by adding 766.8 µL DMSO to the 500 µg vial of JC-1. Any unused 1 mM JC-1 solution should be aliquoted and stored at -20 ºC. 1.4 Working JC-1 Solution: To prepare the Working JC-1 Solution, add the appropriate volume free sign language websiteWebJC-1 Mitochondrial Membrane Potential Detection Kit is used to measure mitochondrial membrane potential changes in cells, a key indicator of cell viability. Features Rapid, … free sign maker downloadWebJul 7, 2024 · JC-1, a cationic fluorescent dye when added to living cells, is known to be localized exclusively in mitochondria, particularly in good physiological conditions characterized by sufficient mitochondrial membrane potential (ΔΨ). The accumulation of JC-1 in these organelles leads to the formation J-ag … freesign pluginWebJC-1 BH-3 profiling is best suited to cell lines or homogenous samples because it provides a bulk response and not a single cell measurement. It is one of the easiest methods to use, … farm store camas waWebWhen excited simultaneously by 488 nm argon-ion laser sources, the JC-1 monomer and J-aggregate can be detected separately in the conventional flow cytometer FL1 and FL2 … farm store cal poly pomonafarm store california