L34963 thermofisher
WebMar 1, 2024 · Live cells were stained with Live/Death violet cell viability staining (L34963; ThermoFisher; Waltham, MA, USA) after which they were fixed and analyzed with a FACSVerse flow cytometer (BD, Franklin Lakes, NJ, USA) and BD Facs Suite software. Immunofluorescence. WebAug 5, 2024 · For all experiments, cells were stained with the LIVE/DEAD Fixable Violet Dead cell stain kit (ThermoFisher, #L34963) following the recommended protocol and only viable cells were included in the analysis. Flow cytometry was performed on a FACSAria II Flow Cytometer machine (BD Biosciences).
L34963 thermofisher
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WebFreshly isolated porcine NK cells were washed with Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) + 1% fetal calf serum (FCS) (Thermo Fisher Scientific) and labeled with a primary mouse mAb for 20 minutes at 4°C. The following primary mAbs were used to identify the presence of CR3 on porcine NK cells: mouse anti-pig CD11R1 … WebBlythewood is a town in Richland and Fairfield counties, South Carolina, United States.It is a suburb of Columbia, South Carolina.The 2024 United States census puts the population at …
WebFeb 19, 2024 · Cells from epithelium, MLN, and spleen ( n = 4/group) were stained with antibodies: Live/Dead fixable violet dead cell stain kit ( L34963, ThermoFisher), AF647 anti-rat TCR αβ (20116, Biolegend, San Diego, CA), FITC anti-rat CD8a (202403, Biolegend), and PE anti-rat TCR γδ (202605, Biolegend). Web细胞功能试剂和试剂盒 LIVE/DEAD™ 可固定紫色死细胞染色试剂盒，用于 405 nm 激发 Use with the ArC™ Amine Reactive Compensation Bead Kit Invitrogen™ LIVE/DEAD™ 可固定紫色死细胞染色试剂盒，用于 405 nm 激发 货号: L34963 相关应用: Cell Viability, Proliferation & Function 技术支持 客户服务 产品概述 图表 推荐产品 文件 常见问题解答 引用和文献 …
Webwww.thermofisher.com. Reactive dye Number of assays (Cat. no.) Excitation source Ex* Em* Blue fluorescent reactive dye 80 (L34961) 200 (L23105) 400 (L34962) UV 350 nm 450 nm … WebMar 11, 2024 · Peli1 is an E3 ubiquitin ligase that mediates both Lys63- and Lys48-linked polyubiquitination of specific protein substrates to modulate immune responses. 6, 7, 8, 9 We previously observed that...
WebCompany Thermo Fisher Scientific. Price Pricing Info Supplier Page View Company Product Page. Catalog Number L34964. Quantity 400 assays. Applications Cell Analysis, Cell …
WebLive cells were stained with Live/Death violet cell viability staining (L34963; ThermoFisher; Waltham, MA, USA) after which they were fixed and analyzed with a FACSVerse flow … enterkinfoot pathWebThe first issue with such dyes is the dependence on a consistent dead cell source for fluorescence compensation. Another major issue is the stability of the staining; except for … dr grann oncologyWebAdditional product information Supplier also known as. Thermo Fisher Scientific, Life Technologies, Pierce, Gibco, Applied Biosystems, Affymetrix, eBioscience, Caltag ... enter keyword or product numberWebFeb 27, 2024 · Using flow cytometric analysis of single cell suspensions prepared from brain tissue and immunohistochemistry, we investigated the potential inflammatory mechanisms by which VWF contributes to... enter key not working on doom consoleWebThermo Fisher l 34963 ebioscience foxp3 transcription factor staining buffer set thermofisher scientific L 34963 Ebioscience Foxp3 Transcription Factor Staining Buffer Set Thermofisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. enter keyword title author or isbnWebCatalog Number L34963 Product Name LIVE/DEAD® Fixable Violet Dead Cell Stain Kit *for 405 nm excitation**80 assays* Lot Number 1921486 LOT DATA SPECIFICATION ASSAY … dr. grannum officeWebFeb 19, 2024 · These microbe-associated molecular patterns (MAMPs) possess immunomodulatory properties. In human, it has been suggested that NK cells can be activated by β-glucans. Here, we aimed to elucidate... enter key sticking on keyboard