2024 Top-flash fop-flash

Top-flash fop-flash

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August undefined, 2024

WebTop和Fop序列基本上一致的，Top的荧光素酶启动子上有几个Lef/TCF的结合位点，而在FOp是突变的，因此Top/Fop可以检测Wnt的活性改变。 1.应该是要用Top/FOP值来表示 … WebFOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or sh-circAGFG1#1 group. h The nuclear translocation of β-catenin in sh-NC or sh-circAGFG1#1 group was assessed by IF assay (scale bar = 20 μm). i Luciferase reporter assay ...

Supplementary Information Materials and Methods - Journal of …

WebSupplementary Information Materials and Methods TOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites … WebJ-STAGE Home frozen four 2023 tampa

β-catenin activation down-regulates cell-cell junction ... - Nature

WebPlasmid TOP-GFP from Dr. Ramesh Shivdasani's lab contains the insert 7xTOP-GFP and is published in Cancer Res. 2012 Feb 8. This plasmid is available through Addgene. ... We inserted the 7xTCF/LEF promoter cassette from the M50 Super-TOP flash plasmid (Randall Moon) into the pRRLSIN.cPPT.PGK-GFP.WPRE vector (Didier Trono), replacing the PGK ... WebTOP/FOP-FLASH assay in HEK293T cells? Question. 7 answers. Asked 29th Aug, 2024; ... TOP or FOP plasmid + Opti-MEM and Lipofectamine + Opti-MEM are incubated at room … Web22. jan 2024 · Visual Difference. Adobe Flash can be a troll for original cartoons trying to pick traction on tv. This includes shows like Fish Hooks, Johnny Test,and Daniel Tiger (kids show). The animation type looks … giants gm 2021

How to detect and activate Wnt signaling The Wnt Homepage

Top Fop Flash Vector Promega Bioz

WebFigure Legend Snippet: a The luciferase activity of Wnt/β-catenin signaling was detected using TOP-flash assay with FOP-flash as negative control. b Western blot of p-GSK-3β, GSK-3β, β-catenin, c-Myc in PTC cells under DOCK9-AS2 depletion. c The nuclear and cytoplasm levels of β-catenin were examined by western blotting. d Luciferase ... Web2.6 TOP/FOP flash assay The wild-type (TOP) and mutant-type (FOP) LEF/TCF reporter genes were cloned to the pGL3 luciferase constructs (Promega Corp., Madi-son, WI, USA). Cells in each group were cultured in 48-well plates at a density of 2 ×104 cells per well for 24 h. Each well was loaded with 0.2 μg TOP/FOP flash and 1 ng Renilla (pRLTK ... frozen four 2023 ticketmasterWeb具体的TOP或FOP质粒与海肾荧光素载体的比例，需要个人通过实验优化，通常取值的范围在10/1到50/1。数据处理：通常以TOP/FOP的比值表示。公式如下：转染有TOP-Flash和海肾荧光素载体的样本中采集得到的萤火虫荧 … frozen four bracket 2022

"Web19. nov 2024 · TOP-flash/FOP-flash luciferase reporter assay. Cells were serum-starved overnight and co-transfected with 200 ng TOP-flash or FOP-flash expression plasmid and 50 ng pRL-TK using Lipofectamine 3000 " - Top-flash fop-flash

Top-flash fop-flash

TOP/FOP-FLASH assay in HEK293T cells? ResearchGate

WebFrom that 50ng DNA, 15ng are TOPFlash plasmid (Firefly), 10 ng Renilla, and the rest empty plasmid (PCS2 or pCDNA3). Treat the cells O/N with concentrations between 1-3uM of GSK3i or with Wnt3a.... Web一般而言，topflash/fopflash是比较常用的检测β-catenin的系统，原理和所有的RLU一样，都是利用转录因子可以和特异性的靶位点结合进而启动下游的虫荧光素酶基因的表达。如 …

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Web23. nov 2024 · The TOP flash/FOP flash dual-luciferase report system was used to detect Wnt signaling pathway activity of the Ad-ROP18- or Ad-transfected C17.2 NSCs. Briefly, C17.2 NSCs were plated at a density of 1 × 10 4 cells/ml in 24-well plates. After 16 h culture, 500 ng TOP flash plasmid (Millipore) containing six TCF-binding motifs and firefly ... WebPrice from $9.99 to $1999.99 luciferase top flash - by Bioz Stars , 2024-04 86 / 100 stars Buy from Supplier luciferase flash assay kit ( Thermo Fisher ) 96 Thermo Fisher luciferase flash assay kit Luciferase Flash Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations.

WebFOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and … WebFOPFlash(报告基因质粒)(D2503-1μg) FOPFlash(报告基因质粒)是TOPFlash(D2501)的阴性对照报告基因质粒。微信在线咨询扫码加微信快速订购联系我们选择语言：中文 …

WebTOPで生じたレポーターの発現がFOPで起きなければ、ベクターからの非特異的な転写誘導ではなく、導入した細胞においてWnt経路のシグナル伝達が生じていると考えられます。 Web3. sep 2024 · TOP/FOP Flash (Genechem) was co-transfected into MHCC97-H, HCCLM3, or Hep 3B cells along with indicated plasmids as needed. The luciferase activity of TOP Flash or FOP Flash was monitored via Dual Luciferase Report Assay System (Promega, Madison, WI, USA). The TOP/FOP ratio was then calculated to assess the activity of Wnt/β-catenin …

WebTo measure Wnt signaling, one can use a variety of Wnt reporters. The TOP-flash assay ( Molenaar 1996) is widely used and variants of TOP-Flash (SuperTop) are available from …

WebTop Fop Flash, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more 86 stars, based on 1999.99 , 2024-04 86 / 100 stars Images top flash fop flash vector ( Promega ) About News Press Release Team Advisors Partners Contact Bioz Stars giants gm newsWebLuciferase assays can be used to investigate a variety of cellular processes using bioluminescent light output as a sensitive measure of biological activity. We offer a full range of luciferase assay products and tools for your research. Reporter options include NanoLuc®, firefly and Renilla luciferases, which have different protein sizes ... frozen four 2023 tvWebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : … frozen four 2023 women\u0027s hockey giants gm news todayWebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : Change media with 0 ... frozen four bracketologyWebThis 134bp fragment was gene synthesized and cloned into the ASP718 and XMA-1 sites of the vector. Note: This plasmid was published as M51 Super 8x FOPFlash, but the plasmid … frozen four bracket predictionsWebTOPflash (TCF Reporter Plasmid) 21-170 Sigma-Aldrich TOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase mi More>> frozen four 2023 wiki