site stats

Topflash/fopflash reporter

Web15. máj 2024 · While all three compounds had comparable inhibitory effect on the TopFlash reporter above 2.5 µM, compounds C11 and C16 also negatively affected the FopFlash reporter, thus indicating... WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF …

SuperTOPFlash-CP(超灵敏快速降解型报告基因质粒)(D2506-1μg)

Web一般而言,topflash/fopflash是比较常用的检测β-catenin的系统,原理和所有的RLU一样,都是利用转录因子可以和特异性的靶位点结合进而启动下游的虫荧光素酶基因的表达。如果 … WebTransfection grade T cell factor (Tcf) reporter plasmid containing three copies of the Tcf binding site (wt) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. FOPFLASH (Catalog # 21-169) containing mutated Tcf binding sites is also available as a negative control. TopFlash Multiple cloning site . TopFlash ... espn women\u0027s history month https://mcmanus-llc.com

Topflash Fopflash Reporter Addgene inc Bioz

WebTo measure Wnt signaling, one can use a variety of Wnt reporters. The TOP-flash assay ( Molenaar 1996) is widely used and variants of TOP-Flash (SuperTop) are available from … WebTOPflash (TCF-Reporterplasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and Luciferase open reading frame.; find Sigma-Aldrich-21170 MSDS, related peer-reviewed papers, … http://www.app17.com/c145959/products/d11302704.html finns hotelhotel gramadomarche hotel

Effects of TC1 on TOP/FOPFLASH analysis. A, TOPFLASH

Category:Assaying β-Catenin/TCF Transcription with β-Catenin/TCF

Tags:Topflash/fopflash reporter

Topflash/fopflash reporter

TopFlash plasmid,TopFlash,TopFlash plasmid,TopFlash sequence,TopFlash …

WebAddgene inc topflash fopflash luciferase reporter plasmids Topflash Fopflash Luciferase Reporter Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold …

Topflash/fopflash reporter

Did you know?

WebArrowed cells showed nuclear staining of β-catenin. ( G ) Huh7 cells were transfected with equal amounts of either Super8xTOPFlash reporter or Super8xFOPFlash reporter constructs. After 48 hours, the TopFlash/FopFlash luciferase activity of CD133 + and CD133 - Huh7 cells was measured. Values shown are relative luminescence. WebKai F. Albring Charité Universitätsmedizin Berlin Hi Jude, monitoring Wnt/beta-catenin activity with Topflash reporters is pretty simple, as long as tranfection is working. There are several...

Web1169.00元. TOPFlash(报告基因质粒)是用于检测Wnt信号通路中β-catenin介导的TCF/LEF转录活性水平的报告基因质粒。. TOPFlash是以碧云天的pGL6-TA为模板,在其多克隆位点插 … WebThis is a CONTROL luciferase reporter with the TCF/LEF sites of Super8XTOPflash (construct M50, Addgene Plasmid #12456) being mutated. There are 6 mutated TCF/LEF …

WebTransfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase mi More>> TOPflash (TCF Reporter Plasmid) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. MSDS

WebZoom Set of transfection grade T cell factor (TCF) reporter plasmids for use in TOPFlash and FOPFlash wnt/b-catenin activity assays. TCF Reporter Plasmid Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. MSDS 分析证书 参考 17-285 1 kit 无法检索价格。 推荐的产品 概述 支持文件 相关产品和应 …

WebReporter Vector primer 4 (RVprimer4) binding region: 3746-3765: ColE1-derived plasmid replication origin: 4003: Ampicillin: 4794-5654: Synthetic poly(A) signal/transcriptional pause site: 5759-5912: Reporter Vector primer 3 (RVprimer3) binding region: 5861-5880 espn women\u0027s tournamentWebTopflash Fopflash Luciferase Reporter Assaythe Topflash Fopflash Reporter Plasmids, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, … finn shortland streetWebThe first transcription-based luciferase reporter of Wnt/β-catenin signaling, TOPFlash, was designed by Korinek et al. . The TOPFlash reporter contains three TCF response elements (CCTTTGATC) upstream of a basal c-fos promoter while the control reporter, FOPFlash, contains three mutant TCF response elements (CCTTTG GC C). TOP-Flash was later ... finnshrimp soundcloudhttp://www.labbase.net/Supply/SupplyItems-4229347.html espn women\u0027s scores basketballWebThis reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is … espn won\u0027t let me watch liveWebSix Best Rslash Podcasts For 2024. Latest was r/Trueoffmychest The Dad I Babysit for is Grooming Me. Listen online, no signup necessary. espn women\u0027s softball rankingsWebDescription. FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three … espn world cup 2026